科研项目:
1)国家自然科学基金面上基金项目,重构里氏木霉纤维素酶系促进人造淀粉的生物合成、主持。
2)国家自然科学基金青年基金项目,调控里氏木霉胞内槐糖积累促进纤维素酶合成、主持。
3)重庆市自然科学基金项目,蛋白质激酶 Hal4 调控里氏木霉胞内离子浓度在纤维素酶基因转录调控中的作用机制,主持。
4)重庆市自然科学基金项目,构建里氏木霉新型基因工程菌高效合成纤维素酶,主持。
5)重庆市教育委员会科学技术研究项目、体外多酶催化玉米秸秆转化淀粉的基础研究,主持。
6)重庆市教育委员会科学技术研究项目,构建低成本微藻培养体系的应用基础研究,主持。
7) 福建省海洋藻类活性物质制备与功能开发重点实验室开放课题、海洋小球藻多重基因编辑系统构建,主持。
8)智能制造服务国际科技合作基地开放项目,基于微生态分析分散式污水处理系统运营管理优化,主持。
第一和通讯作者文章:
1) YD Chen, YS Gao, ZC Wang, N Peng, XQ Ran, TT Chen, LL Liu, YH Li*. The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers. Fermentation 2023, 9(8), 746.
2)JH Li, YD Chen, YS Gao, Y Mo, TT Long, B Yao, YH Li*. Engineering Trichoderma reesei for the hyperproduction of cellulose induced protein 1 (Cip1) on a sophorose-containing inducer to efficiently saccharify alkali-pretreated corn stover. Prep Biochem Biotech, 2023, 53(7):880-890.
3)YD Chen, N Peng, YS Gao, Q Li, ZC Wang, B Yao, YH Li*. Two-stage pretreatment of Jerusalem artichoke stalks with wastewater recycling and lignin recovery for the biorefinery of lignocellulosic biomass. Processes, 2023, 11(1), 127.
4)YH Li, Peng Zhang, DY Zhu, B Yao, T Hasunuma, A Kondo, XQ Zhao. Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes. Biochemical Engineering Journal, 2022, 178, 108296.
5)TT Long, P Zhang, JZ Yu, YS Gao, XQ Ran, YH Li*. Regulation of β-Disaccharide Accumulation by β-Glucosidase Inhibitors to Enhance Cellulase Production in Trichoderma reesei. Fermentation 2022, 8, 232.
6) P Zhang, Q Li, YD Chen, N Peng, WS Liu, XM Wang, YH Li*. Induction of cellulase production in Trichoderma reesei by a glucose-sophorose mixture as an inducer prepared using stevioside. RSC Advances, 2022, 12, 17392-17400.
7)YH Li*, JZ Yu, Peng Zhang, Tingting Long, Yi Mo, JH Li, Qian Li. Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide. Bioresources and Bioprocessing, 2021, 1, 57.
8)YH Li, XY Zhang, F Zhang, LC Peng, DB Zhang, A Kondo, FW Bai, XQ Zhao. Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover. Biotechnology for Biofuels, 2018, 11, 49.
9)YH Li, XY Zhang, L Xiong, MA Mehmood, XQ Zhao, FW Bai. On-site cellulase production and efficient saccharification of corn stover employing cbh2 overexpressing Trichoderma reesei with novel induction system. Bioresource Technology, 2017, 238, 643-649.
10)YH Li, CG Liu, FW Bai, XQ Zhao. Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding synthesized mixture composed of glucose and disaccharides as substrate and inducer for biorefinery. Bioresource Technology, 2016, 216: 503-510.